Sabiia Seb
PortuguêsEspañolEnglish
Embrapa
        Busca avançada

Botão Atualizar


Botão Atualizar

Ordenar por: 

RelevânciaAutorTítuloAnoImprime registros no formato resumido
Registros recuperados: 160
Primeira ... 12345678 ... Última
Imagem não selecionada

Imprime registro no formato completo
Genetic differentiation and estimated levels of gene flow in members of the Gammarus pulex-group (Crustacea, Amphipoda) in western Europe Naturalis
Scheepmaker, Maarten.
Genetic differentiation of morphologically variable members of the G. pulex-group in northwestern Europe was investigated by electrophoresis at 20 enzyme loci. Five recently described related forms were examined with reference to the “classical” species G. pulex, G. wautieri and G. fossarum. G. pulex and G. wautieri were shown to be genetically homogeneous and clearly distinct species. In the other species, morphologically similar forms were shown to be genetically distant, and inter-areal intraspecific genetic differentiation may warrant recognition of subspecies or sibling species. In some cases, levels of reproductive isolation of the forms involved could be assessed by cross-breeding experiments. To estimate dispersal capabilities, gene flow levels...
Tipo: Article / Letter to the editor Palavras-chave: Taxonomy; Distribution; Population structure; Intra-specific divergence; Gammarus.
Ano: 1990 URL: http://www.repository.naturalis.nl/record/504326
Imagem não selecionada

Imprime registro no formato completo
Genetic diversity and population structure analysis of strawberry (Fragaria x ananassa Duch.) using SSR markers Electron. J. Biotechnol.
Yoon,Min-Young; Moe,Kyaw Thu; Kim,Dae-Young; Rho,Il-Rae; Kim,Su; Kim,Ki-Taek; Won,Mi-Kyoung; Chung,Jong-Wook; Park,Yong-Jin.
In total, 18 simple sequence repeat (SSR) markers were used to analyze the genetic diversity and population structure of 59 accessions of cultivated strawberry (Fragaria x ananassa Duch.) from Korea, Germany, United States, United Kingdom, and Japan. In total, 101 alleles were detected with an average of 5.6 per locus and 21 specific alleles were identified. Notably, one genotype (Blonoli from Germany) possessed a maximum of 10 different unique alleles specific to each genotype. The gene diversity varied from 0.027 (EMPaEKO1B) to 0.791 (CFACT110), with an average value of 0.509. PIC values ranged from 0.026 to 0.762 (average 0.454). A model-based structure analysis revealed the presence of two populations. The accessions that were clearly assigned to a...
Tipo: Journal article Palavras-chave: Genetic diversity; Population structure; SSR markers; Strawberry.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000200006
Imagem não selecionada

Imprime registro no formato completo
Genetic diversity and population structure of the Guinea pig (Cavia porcellus, Rodentia, caviidae) in Colombia Genet. Mol. Biol.
Burgos-Paz,William; Cerón-Muñoz,Mario; Solarte-Portilla,Carlos.
The aim was to establish the genetic diversity and population structure of three guinea pig lines, from seven production zones located in Nariño, southwest Colombia. A total of 384 individuals were genotyped with six microsatellite markers. The measurement of intrapopulation diversity revealed allelic richness ranging from 3.0 to 6.56, and observed heterozygosity (Ho) from 0.33 to 0.60, with a deficit in heterozygous individuals. Although statistically significant (p < 0.05), genetic differentiation between population pairs was found to be low. Genetic distance, as well as clustering of guinea-pig lines and populations, coincided with the historical and geographical distribution of the populations. Likewise, high genetic identity between improved and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Food security; Microsatellite; Population structure.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400027
Imagem não selecionada

Imprime registro no formato completo
Genetic diversity, multiplicity of infection and population structure of Schistosoma mansoni isolates from human hosts in Ethiopia ArchiMer
Aemero, Mulugeta; Boissier, Jerome; Climent, Deborah; Mone, Helene; Mouahid, Gabriel; Berhe, Nega; Erko, Berhanu.
Background: Human intestinal schistosomiasis caused by Schistosoma mansoni and urinary schistosomiasis caused by Schistosoma haematobium are endemic in Ethiopia. Although schistosomes look morphologically uniform, there is variation in infectivity, egg productivity and virulence due to variation in their genetic make. Knowing the genetic diversity and population structure of S. mansoni isolates will enable to understand and consider the possible variability in terms of infectivity, egg productivity and virulence. Methods: Between 2010 and 2011, genetic diversity and population structure of Schistosoma mansoni isolates from four endemic areas of Ethiopia was assessed using previously published 11 polymorphic microsatellite loci. Miracidia were hatched from...
Tipo: Text Palavras-chave: Schistosoma mansoni; Microsatellite; Genetic diversity; Population structure; Ethiopia.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00302/41369/71868.pdf
Imagem não selecionada

Imprime registro no formato completo
Genetic diversity of Morato's Digger Toad, Proceratophrys moratoi: spatial structure, gene flow, effective size and the need for differential management strategies of populations Genet. Mol. Biol.
Arruda,Mauricio P.; Costa,William P.; Recco-Pimentel,Shirlei M..
Abstract The Morato's Digger Toad, Proceratophrys moratoi, is a critically endangered toad species with a marked population decline in southern Brazilian Cerrado. Despite this, new populations are being discovered, primarily in the northern part of the distribution range, which raises a number of questions with regard to the conservation status of the species. The present study analyzed the genetic diversity of the species based on microsatellite markers. Our findings permitted the identification of two distinct management units. We found profound genetic structuring between the southern populations, on the left margin of the Tietê River, and all other populations. A marked reduction was observed in the contemporary gene flow among the central populations...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Proceratophrys moratoi; Microsatellite; Population structure; Management unit; Conservation.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300502
Imagem não selecionada

Imprime registro no formato completo
Genetic structure of the snakehead murrel, Channa striata (channidae) based on the cytochrome c oxidase subunit I gene: influence of historical and geomorphological factors Genet. Mol. Biol.
Jamaluddin,Jamsari Amirul Firdaus; Pau,Tan Min; Siti-Azizah,Mohd Nor.
Nucleotide sequences of a partial cytochrome c oxidase subunit I gene were used to assess the manner in which historical processes and geomorphological effects may have influenced genetic structuring and phylogeographic patterns in Channa striata. Assaying was based on individuals from twelve populations in four river systems, which were separated into two regions, the eastern and western, of the biodiversely rich state of Perak in central Peninsular Malaysia. In 238 specimens, a total of 368-bp sequences with ten polymorphic sites and eleven unique haplotypes were detected. Data on all the twelve populations revealed incomplete divergence due to past historical coalescence and the short period of separation. Nevertheless, SAMOVA and F ST revealed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Channa striata; MtDNA COI; Phylogeography; Population structure.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100026
Imagem não selecionada

Imprime registro no formato completo
Genetic variability of two populations of Pseudoplatystoma reticulatum from the Upper Paraguay River Basin Genet. Mol. Biol.
Abreu,Marcia Matos de; Pereira,Luiz Henrique Garcia; Vila,Vilma Barretto; Foresti,Fausto; Oliveira,Claudio.
Catfishes of the genus Pseudoplatystoma are very important species due to both their high commercial value and their ecological role as voracious predators. They undertake lengthy migratory movements during their life-cycle, this including reproductive migration which occurs from October to December in the rainy season. In the present study, seven microsatellite loci were analyzed to access genetic variability in two samples of P. reticulatum from the Upper Paraguay Basin. The loci were highly polymorphic (mean = 7.28). According to all analysis, the two samples of P. reticulatum revealed pronounced genetic differentiation. Fst value was 0.2290, Rst value 0.1067 and AMOVA 22.90% (Fst) and 10.67% (Rst), all being highly significant (p < 0.001). The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fish; Population genetics; Microsatellite; Homing; Population structure.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400032
Imagem não selecionada

Imprime registro no formato completo
Genetics, evolution and conservation of Bromeliaceae Genet. Mol. Biol.
Zanella,Camila M.; Janke,Aline; Palma-Silva,Clarisse; Kaltchuk-Santos,Eliane; Pinheiro,Felipe G.; Paggi,Gecele M.; Soares,Luis E.S.; Goetze,Márcia; Büttow,Miriam V.; Bered,Fernanda.
Bromeliaceae is a morphologically distinctive and ecologically diverse family originating in the New World. Three centers of diversity, 58 genera, and about 3,140 bromeliad species are currently recognized. We compiled all of the studies related to the reproductive biology, genetic diversity, and population structure of the Bromeliaceae, and discuss the evolution and conservation of this family. Bromeliads are preferentially pollinated by vertebrates and show marked variation in breeding systems, from predominant inbreeding to predominant outcrossing, as well as constancy in chromosome number (2n = 2x = 50). Autogamous or mixed mating system bromeliads have a high inbreeding coefficient (F IS), while outcrossing species show low F IS. The degree of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bromeliads; Cytogenetics; Genetic diversity; Population structure; Reproductive biology.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600017
Imagem não selecionada

Imprime registro no formato completo
Growth parameters of the invasive gastropod Melanoides tuberculata (Müller, 1774) (Gastropoda, Thiaridae) in a semi-arid region, Northeastern Brazil Biological Sciences
Oliveira, Cicero Diogo Lins; Oliveira, Carlos Yure Barbosa de.
Melanoides tuberculata has invaded several aquatic ecosystems in the Neotropical region. This species shows high adaptive capacity and plasticity, establishing itself in several basins, promoting negative impacts on environments and native species. Here, we determine the growth parameters and the population structure of this species, in a semi-arid environment, in Northeast Brazil (Pernambuco state). Monthly captures were conducted at three types of substrate: site 1: soft bottom with macrophytes, site 2: soft bottom without macrophytes, and site 3: gravel and rocks. Shells were classified as occupied or empty, and their shell length (SL) and opening width (SW) were measured. The growth parameters of the von Bertalanffy’s model were estimated from the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Invasive mollusk; Population structure; Growth curve. Zoologia.
Ano: 2019 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/45720
Imagem não selecionada

Imprime registro no formato completo
High interpopulation homogeneity in Central Argentina as assessed by Ancestry Informative Markers (AIMs) Genet. Mol. Biol.
García,Angelina; Dermarchi,Darío A.; Tovo-Rodrigues,Luciana; Pauro,Maia; Callegari-Jacques,Sidia M.; Salzano,Francisco M.; Hutz,Mara H..
The population of Argentina has already been studied with regard to several genetic markers, but much more data are needed for the appropriate definition of its genetic profile. This study aimed at investigating the admixture patterns and genetic structure in Central Argentina, using biparental markers and comparing the results with those previously obtained by us with mitochondrial DNA (mtDNA) in the same samples. A total of 521 healthy unrelated individuals living in 13 villages of the Córdoba and San Luis provinces were tested. The individuals were genotyped for ten autosomal ancestry informative markers (AIMs). Allele frequencies were compared with those of African, European and Native American populations, chosen to represent parental contributions....
Tipo: Info:eu-repo/semantics/article Palavras-chave: AIMs; Córdoba; San Luis; Migration; Population structure.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300324
Imagem não selecionada

Imprime registro no formato completo
Importance and future of individual markers for the ecosystem approach to fisheries ArchiMer
Fromentin, Jean-marc; Ernande, Bruno; Fablet, Ronan; De Pontual, Helene.
The use of genetic, biochemical and electronic markers in population biology and ecology has been growing tremendously during the last two decades. The first part of this paper aims at reviewing the main principles and advances of these individual markers through a few key applications on exploited marine fish populations. The second part is more prospective and investigates some possibilities that could arise in the near future through: (i) the development of new markers, (ii) the combination of different markers and (iii) the combination of quantitative approaches-whether classical or new-with individual markers. It is finally stressed how crucial individual markers will be to unravel the biocomplexity of wild fish populations and the key role they...
Tipo: Text Palavras-chave: Genetic marker; Biochemical marker; Electronic tag; Otolith; Isotope; Population structure; Statistical and mechanistic model; Fisheries.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/00000/11163/7522.pdf
Imagem não selecionada

Imprime registro no formato completo
Influencia ambiental en la distribución espacial de las clases de edad de pez palo (Percophis brasiliensis) en el Sistema Costero del Atlántico Sudoccidental (34°-41° S) OceanDocs
Barretto, A.C..
The age-class distribution of the Brazilian flathead Percophis brasiliensis in relation to environmental variables in spring and winter, and the growth of both sexes, were studied in the SW Atlantic Coastal System (SACS)(34°S-31° S). Growth was studied from 1449 individuals (n=853, spring (1998 + 2000), n=596, winter (2004)) to which size (TL), sex, and age were determined. Age was determined considering that an opaque plus a hyaline band (present in the transverse section of the sagitta otolith) represented 1 year of life. The age-class distribution in spring and winter was established through hierarchical clustering and non-metric multi-dimensional scaling (nMDS analysis). This analysis grouped sampling stations (n=109, spring 1998; n=160, winter 2004)...
Tipo: Theses and Dissertations Palavras-chave: Age groups; Age composition; Environmental factors; Geographical distribution; Population structure; Growth; Environmental effects; Coastal waters; Age composition; Environmental factors; Geographical distribution; Population structure; Growth; Age groups; Coastal waters; Http://aims.fao.org/aos/agrovoc/c_29093; Http://aims.fao.org/aos/agrovoc/c_2594; Http://aims.fao.org/aos/agrovoc/c_5083; Http://aims.fao.org/aos/agrovoc/c_6115; Http://aims.fao.org/aos/agrovoc/c_3394; Http://aims.fao.org/aos/agrovoc/c_28628; Http://aims.fao.org/aos/agrovoc/c_25205.
Ano: 2007 URL: http://hdl.handle.net/1834/3017
Imagem não selecionada

Imprime registro no formato completo
Informe sobre el muestreo de desembarque de anchoíta (Engraulis anchoita) y caballa (Scomber japonicus) en el puerto de Mar del Plata: período septiembre 1999-enero 2000, con algunos comentarios sobre el manejo de estos recursos. OceanDocs
Garciarena, A.D.; Perrotta, R.G.; López, F..
The chub mackerel Scomber japonicus and anchovy Engraulis anchoita commercial landings at the Mar del Plata (Buenos Aires prov., Argentina) seaport during the September 1999-January 2000 period are studied. A total of 14 chub mackerel samples were taken for total length measurements exclusively and 5 anchovy sub-samples and 1 chub mackerel sub-sample were analyzed to record individual data total length, weight, sex, sexual stage, age from otoliths readings . Most of the fish caught for both species were adults and only a small fraction of chub mackerel juveniles was found in December 1999. Length-at-fifty-percent-maturity was calculated, length-weight and length-age relationships were fitted and length growth parameters estimated. Sea surface temperature...
Tipo: Journal Contribution Palavras-chave: Commercial fishing; Population structure; Catch/effort; Size distribution; Mackerel fisheries; Population structure; Http://aims.fao.org/aos/agrovoc/c_6115.
Ano: 2002 URL: http://hdl.handle.net/1834/2545
Imagem não selecionada

Imprime registro no formato completo
Interethnic admixture and the evolution of Latin American populations Genet. Mol. Biol.
Salzano,Francisco Mauro; Sans,Mónica.
A general introduction to the origins and history of Latin American populations is followed by a systematic review of the data from molecular autosomal assessments of the ethnic/continental (European, African, Amerindian) ancestries for 24 Latin American countries or territories. The data surveyed are of varying quality but provide a general picture of the present constitution of these populations. A brief discussion about the applications of these results (admixture mapping) is also provided. Latin American populations can be viewed as natural experiments for the investigation of unique anthropological and epidemiological issues.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Interethnic admixture; Latin America; Population structure.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000200003
Imagem não selecionada

Imprime registro no formato completo
Intraspecific variation and population structure of the Velvetbean Caterpillar, Anticarsia gemmatalis Hübner, 1818 (Insecta: Lepidoptera: Noctuidae) Genet. Mol. Biol.
Sosa-Gómez,Daniel R..
The velvetbean caterpillar (VBC), Anticarsia gemmatalis Hübner, 1818 (Insecta: Lepidoptera: Noctuidae), is one of the most important New World soybean agro-ecosystems pests, occurring from 40° N in the USA to 39° S in Argentina. Information on the migration patterns of the VBC moth may be important for managing the resistance of VBC populations to insecticides or plants carrying the Bacillus thuringiensis insecticide genes, especially since sedentary populations have a higher potential to became resistant than migratory populations. We studied intraspecific variations of geographically distinct VBC populations in order to determine the genetic distance between them and to assess the variability of VBC populations from near the city of Londrina (Paraná (PR)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Molecular marker; RAPD; Gene flow; Soybean pest; Population structure.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300012
Imagem não selecionada

Imprime registro no formato completo
JAMARC-INIDEP joint research cruise on Argentine short-finned squid Illex argentinus. January-March 1997. OceanDocs
Brunetti, N.E.; Ivanovic, M.L.; Rossi, G.R.; Elena, B.; Benavides, H.R.; Guerrero, R.A.; Blanco, G.; Marchetti, C.; Piñero, R..
The distribution, abundance and population structure of Illex argentinus in relation to the oceanographic conditions during summer 1997, were analyzed as a result of the research cruise carried out by R/V Shinko Maru No.3 on the Argentine shelf (39-50°30'S). Besides, abundance and biological features of Ommastrephes bartrami are analyzed. The main activities carried out during the cruise included jigging operations, tagging (only for I. argentinus) and biological sampling. In addition, meteorological and oceanographic data were collected. A total of 30,2000 individuals were tagged and released. The recapture rate was 0.156. (47 recoveries/30,200 released), considerably higher than that of 1996 (0.030; 4 recoveries/13,191 released). I. argentinus was...
Tipo: Journal Contribution Palavras-chave: Fishery surveys; Abundance; Population structure; Tagging; Cruise reports; Population structure; Tagging; Http://aims.fao.org/aos/agrovoc/c_6115; Http://aims.fao.org/aos/agrovoc/c_14775.
Ano: 2000 URL: http://hdl.handle.net/1834/2537
Imagem não selecionada

Imprime registro no formato completo
Karyotypic polymorphism and evolution within and between the Liolaemus monticola (Iguanidae) "northern 2n = 38-40" chromosome race populations in central Chile RChHN
LAMBOROT,MADELEINE.
Chromosomal genotypes were scored from 359 Liolaemus monticola lizards of the "northern, 2n = 38-40" chromosomal race from 21 locality samples between the Maipo (and one of its tributaries) and the Aconcagua (and one of its tributaries) ribers, plus a sample from the interracial hybridization zone, and some representative locality samples of the "southern 2n = 34" and the "multiple fission 2n = 42-44" chromosomal races for comparisons. The first seven variable chromosomal pairs were coded as Mendelian genotypes and statistically summarized by several clustering and population genetic algorithms. Spatial and temporal differentiation was assessed by chromosome frequencies, chromosomal diversity and heterozygosity. While no differentiation was found for...
Tipo: Journal article Palavras-chave: Iguanidae; Liolaernus monticola; Chromosomal races; "northern 2n = 38-40" chromosomal race; Population structure; Speciation.
Ano: 2001 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2001000000017
Imagem não selecionada

Imprime registro no formato completo
La Baie de Seine: Peuplements et especes benthiques en regime perturbe ArchiMer
Cabioch, L; Retiere, C.
The waters of the Seine River are polluted by urban, industrial and agricultural effluents. In addition direct industrial wastes inputs are emptied into the estuary. Within the limits of the Seine Bay program it was not possible to research pollution impacts on every estuarine ecosystem link; so only benthos have been studied. To take time response of organisms into account, the authors have defined indicators or quantitative evaluation of parameters, reflecting physiological state. They have studied population fluctuations caused by natural or man induced effects.
Tipo: Text Palavras-chave: Hydrodynamics; Population dynamics; Population structure; Bioaccumulation; Pollution effects; Marine pollution; Ecosystem disturbance; Benthos.
Ano: 1985 URL: http://archimer.ifremer.fr/doc/1985/acte-1304.pdf
Imagem não selecionada

Imprime registro no formato completo
La caballa (Scomber japonicus) y las condiciones ambientales en el area bonaerense de El Rincón (39°- 40°30' S), agosto 1996. OceanDocs
Perrotta, R.G.; Madirolas, A.; Viñas, M.D.; Akselman, R.; Guerrero, R.A.; Sánchez, M.F.; López, F.; Castro Machado, F.; Macchi, G.J..
In August 1996 acoustic methods were employed to estimate mackerel (Scomber japonicus) abundance in the Buenos Aires, Argentina shelf area of "El Rincón" (4,287 mn2), located between 39°- 40° 30' S. Results showed a biomass estimate of 77,260 t. ASIMRAD EK-500 equipment operated with a 38 KHz split-beam transducer was used. Midwater fishing trawls were employed to identify echotraces and to collect mackerel samples.Trawls 4 and 5 were the most successful. Samples included only adult fish (310-470 mm Lt and ages ranging 2-11). Length and weight growth parameters and mortality rates were estimated. Analysis of the stomach contents revealed that mackerel was feeding exclusively on zooplankters over 2 mmlength. The analysis of the characteristics (dimensions...
Tipo: Journal Contribution Palavras-chave: Abundance; Biomass; Population structure; Zooplankton; Environmental conditions; Echo surveys; Biomass; Population structure; Zooplankton; Environmental conditions; Http://aims.fao.org/aos/agrovoc/c_926; Http://aims.fao.org/aos/agrovoc/c_6115; Http://aims.fao.org/aos/agrovoc/c_15490; Http://aims.fao.org/aos/agrovoc/c_32980.
Ano: 1999 URL: http://hdl.handle.net/1834/2529
Imagem não selecionada

Imprime registro no formato completo
La décadence d'une ville précoloniale du Togo, Aného, en pays Guin OceanDocs
Dovi, Q.; Nyassogbo, G..
Tipo: Journal Contribution Palavras-chave: Pays guin; Aného; Coastal zone; Colonization; Environmental conditions; Environmental impact; Nutritive value; Population density; Population structure; Product development; Rivers; Environmental impact; Nutritive value; Population structure; Rivers; Environmental conditions; Population density; Product development; Http://aims.fao.org/aos/agrovoc/c_24420; Http://aims.fao.org/aos/agrovoc/c_5278; Http://aims.fao.org/aos/agrovoc/c_6115; Http://aims.fao.org/aos/agrovoc/c_6617; Http://aims.fao.org/aos/agrovoc/c_32980; Http://aims.fao.org/aos/agrovoc/c_6112; Http://aims.fao.org/aos/agrovoc/c_16018.
Ano: 2003 URL: http://hdl.handle.net/1834/1067
Registros recuperados: 160
Primeira ... 12345678 ... Última
 

Empresa Brasileira de Pesquisa Agropecuária - Embrapa
Todos os direitos reservados, conforme Lei n° 9.610
Política de Privacidade
Área restrita

Embrapa
Parque Estação Biológica - PqEB s/n°
Brasília, DF - Brasil - CEP 70770-901
Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco

Valid HTML 4.01 Transitional